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Super-sensitive, sensitive, and super-sensitive and sensitive L-aptamers on one chip.
Double-stranded DNA (dsDNA) aptamers are short single-stranded nucleic acids with high affinity and specificity. Each of these properties has been used for a variety of applications, such as sensing and medical diagnostics. Recently, the sensitivity of L-aptamers was improved using various approaches. However, most of these methods that amplify L-aptamers have a trade-off between sensitivity and specificity. Here, we developed a method to improve both the sensitivity and the specificity of L-aptamers by using a single modified L-strand overhang on both ends. Each modified L-strand contains DNA bases with fluorophores and quenchers at its 5′ and 3′-ends, respectively. We confirmed that the super-sensitive L-aptamer and the double-stranded DNA can be easily distinguished from each other by the fluorescence “on/off” switches. The highly sensitive L-aptamer and the double-stranded DNA can be differentiated from each other by both the fluorescence “on/off” switches and the loop